Frown lines (glabellar lines) can be caused by repeated muscle movement of two muscles- the procerus muscle and corrugator supercilii when you frown or smile often (or both). The procerus muscle contractions will cause bunching of the skin and the appearance of horizontal lines across your nose bridge. The corrugator supercilli muscle contractions will draw the eyebrows together thus creating a few vertical lines between your eyes. When this happens repeatedly over time, the lines may become ingrained or etched in.
Other factors that can contribute to frown line development are sun damage, smoking, and loss of skin elasticity as we get older (these factors also contribute to crow's feet, horizontal forehead lines, and horizontal creases).