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In my opinion, for the best survival of the fat, the following are some principles that might help:
Summary
In my opinion fat transfer or fat grafting is a safe and effective way to create a large volume of fat in areas that need it. However, more studies are needed to establish the best practice to get the best results for the patients.
References: 1. Asilian A, Siadat AH, Iraji R. Comparison of fat maintenance in the face with centrifuge versus filtered and washed fat. J Res Med Sci. 2014 Jun;19(6):556-61. 2. Davis K, Rasko Y, Oni G, Bills J, Geissler P, Kenkel JM. Comparison of adipocyte viability and fat graft survival in an animal model using a new tissue liquefaction liposuction device vs standard Coleman method for harvesting. Aesthet Surg J. 2013 Nov 1;33(8):1175-85 3. Smith P, Adams WP Jr, Lipschitz AH, Chau B, Sorokin E, et al. Autologous human fat grafting: effect of harvesting and preparation techniques on adipocyte graft survival. Plast Reconstr Surg. 2006 May;117(6): 1836-44. 4. Gupta R, Brace M, Taylor SM, Bezuhly M, Hong P. In search of the optimal processing for fat grafting. J Craniofac Surg. 2015 Jan;26(1):94-9. 5. Gerth DJ, King B, Rabach L, Glasgold RA, Glasgold MJ. Long-term volumetric retention of autologous fat grafting processed with closed-membrane filtration. Aesthet Surg J. 2014 Sept:34(7):985-94